S. Dzhindzhev, Nikola Tzolovsky, George Lipinszki, Zoltan Abdelaziz, Mohammed Debski, Janus Dadlez, Michal Glover, David M. GST-Ana2-S38A can bind Sas6 after Plk4 phosphorylation from Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation Supplementary Figure S3: GST-Ana2-S38A can bind Sas6 after Plk4 phosphorylation. Sas6 specifically interacts with both Ana2-WT and Ana2-S38A when the proteins are pre-phosphorylated by Plk4. GST, GST-tagged wild-type (GST-Ana2-WT) or the S38A substitution mutant (GST-Ana2-S38A) were treated with either MBP-Plk4 or MBP-Plk4KD and incubated in vitro with 35S-Met-labeled Sas6 produced in a coupled in vitro transcription/translation reaction. The resulting complex was analyzed by SDS-PAGE and autoradiography. Ana2;Plk4;Sas6;centriole;centrosome 2017-12-08
    https://rs.figshare.com/articles/journal_contribution/GST-Ana2-S38A_can_bind_Sas6_after_Plk4_phosphorylation_from_Two-step_phosphorylation_of_Ana2_by_Plk4_is_required_for_the_sequential_loading_of_Ana2_and_Sas6_to_initiate_procentriole_formation/5683933
10.6084/m9.figshare.5683933.v1