10.6084/m9.figshare.5345971.v1
Hugo J. de Boer
Hugo
J. de Boer
Abdolbaset Ghorbani
Abdolbaset
Ghorbani
Vincent Manzanilla
Vincent
Manzanilla
Ancuta-Cristina Raclariu
Ancuta-Cristina
Raclariu
Anna Kreziou
Anna
Kreziou
Sarawut Ounjai
Sarawut
Ounjai
Maslin Osathanunkul
Maslin
Osathanunkul
Barbara Gravendeel
Barbara
Gravendeel
De Boer et al. DNA metabarcoding of orchid-derived products reveals widespread illegal orchid trade. Proceedings of the Royal Society B. DOI: 10.1098/rspb.2017-1182. Figure S1
The Royal Society
2017
CITES
DNA metabarcoding
food safety
forensic botany
orchids
salep
2017-08-25 07:59:16
Journal contribution
https://rs.figshare.com/articles/journal_contribution/De_Boer_et_al_DNA_metabarcoding_of_orchid-derived_products_reveals_widespread_illegal_orchid_trade_Proceedings_of_the_Royal_Society_B_DOI_10_1098_rspb_2017-1182_Figure_S1/5345971
In eastern Mediterranean countries orchids continue to be collected from the wild for the production of salep, a beverage made of dried orchid tubers. In this study we used nrITS1 and nrITS2 DNA metabarcoding to identify orchid and other plant species present in 55 commercial salep products purchased in Iran, Turkey, Greece and Germany. Thirty samples yielded a total of 161 plant taxa, and 13 products (43%) contained orchid species and these belonged to 10 terrestrial species with tuberous roots. Another 70% contained the substitute ingredient <i>Cyamopsis tetraganoloba</i> (Guar). DNA metabarcoding using the barcoding markers nrITS1 and nrITS2 shows the potential of these markers and approach for identification of species used in salep products. The analysis of interspecific genetic distances between sequences of these markers for the most common salep orchid genera shows that species level identifications can be made with a high level of confidence. Understanding the species diversity and provenance of salep orchid tubers will enable the chain of commercialization of endangered species to be traced back to the harvesters and their natural habitats, and thus allow for targeted efforts to protect or sustainably use wild populations of these orchids.