10.6084/m9.figshare.5203201.v1 Steven M. Driever Steven M. Driever Andrew J. Simkin Andrew J. Simkin Saqer Alotaibi Saqer Alotaibi Stuart J. Fisk Stuart J. Fisk Pippa J. Madgwick Pippa J. Madgwick Caroline A. Sparks Caroline A. Sparks Huw D. Jones Huw D. Jones Tracy Lawson Tracy Lawson Martin A. J. Parry Martin A. J. Parry Christine A. Raines Christine A. Raines Data file 5 from Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions The Royal Society 2017 sedoheptulose-1 7-biphosphatase Calvin–Benson cycle transgenic biomass yield 2017-07-13 09:49:42 Dataset https://rs.figshare.com/articles/dataset/Data_file_5_from_Increased_SBPase_activity_improves_photosynthesis_and_grain_yield_in_wheat_grown_in_greenhouse_conditions/5203201 To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin–Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a <i>Brachypodium dystachion</i> SBPase gene construct. Transgenic lines with increased SBPase protein levels and activity were grown under greenhouse conditions and showed enhanced leaf photosynthesis and increased total biomass and dry seed yield. This showed the potential of improving yield potential by increasing leaf photosynthesis in a crop species such as wheat. The results are discussed with regards to future strategies for further improvement of photosynthesis in wheat.This article is part of the themed issue ‘Enhancing photosynthesis in crop plants: targets for improvement’.