10.6084/m9.figshare.4769527.v1
Leonor Ramirez
Leonor
Ramirez
Pedro Negri
Pedro
Negri
Laura Sturla
Laura
Sturla
Lucrezia Guida
Lucrezia
Guida
Tiziana Vigliarolo
Tiziana
Vigliarolo
Matías Maggi
Matías
Maggi
Martín Eguaras
Martín
Eguaras
Elena Zocchi
Elena
Zocchi
Lorenzo Lamattina
Lorenzo
Lamattina
Electronic Supplementary Material - Ramirez et al.pdf from Abscisic acid enhances cold tolerance in honeybee larvae
The Royal Society
2017
Apis mellifera
cold tolerance
abscisic acid
compensatory growth
2017-03-21 10:05:11
Journal contribution
https://rs.figshare.com/articles/journal_contribution/Electronic_Supplementary_Material_-_Ramirez_et_al_pdf_from_Abscisic_acid_enhances_cold_tolerance_in_honeybee_larvae/4769527
The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in honeybee (<i>Apis mellifera</i>). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in <i>in vitro</i>-reared larvae exposed to low temperatures. Four-days-old larvae (L4) exposed to 25°C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (34°C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 34°C, suggesting a biological significance for ABA. Larvae fed with ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate the adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: <i>Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin</i> and <i>Heat Shock Proteins 70.</i> AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee's larvae to low temperatures through priming effects.