%0 Journal Article
%A Roux, Anne-Laure
%A Viljoen, Albertus
%A Bah, Aïcha
%A Simeone, Roxane
%A Bernut, Audrey
%A Laencina, Laura
%A Deramaudt, Therese
%A Rottman, Martin
%A Gaillard, Jean-Louis
%A Majlessi, Laleh
%A Brosch, Roland
%A Girard-Misguich, Fabienne
%A Vergne, Isabelle
%A de Chastellier, Chantal
%A Kremer, Laurent
%A Herrmann, Jean-Louis
%D 2016
%T Sup. Figure 1: Preferential location of S variants of M. abscessus in loner phagosomes within BMDM.; Sup. Figure 2: In vitro growth of the S and R variants of M. abscessus.; Sup. Figure 3: Comparative intracellular growth of the S (A) and R (B) variants in wild type and ΔF508 murine Mф respectively.; Sup. Figure 4: M. abscessus S variant is able to damage the phagosome membrane of THP-1 cells as assessed by FRET.
%U https://rs.figshare.com/articles/journal_contribution/Sup_Figure_1_Preferential_location_of_S_variants_of_M_abscessus_in_loner_phagosomes_within_BMDM_Sup_Figure_2_In_vitro_growth_of_the_S_and_R_variants_of_M_abscessus_Sup_Figure_3_Comparative_intracellular_growth_of_the_S_A_and_R_B_variants_in_wild_type_and_/4220280
%R 10.6084/m9.figshare.4220280.v1
%2 https://rs.figshare.com/ndownloader/files/6884553
%K Mycobacterium abscessus
%K macrophages
%K phagosome
%K innate response
%K rapid-growing mycobacteria
%X Mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with Mendelian disorders, such as cystic fibrosis (CF). Mycobacterium abscessus is known to transition from a smooth (S) morphotype with cell surface-associated glycopeptidolipids (GPL) to a rough (R) morphotype lacking GPL. Herein, we show that M. abscessus S and R variants are able to grow inside macrophages and are present in morphologically distinct phagosomes. The S forms are found mostly as single bacteria within phagosomes characterized by a tightly apposed phagosomal membrane and the presence of an electron translucent zone (ETZ) surrounding the bacilli. By contrast, infection with the R form leads to phagosomes often containing more than two bacilli, surrounded by a loose phagosomal membrane and lacking the ETZ. In contrast with the R variant, the S variant is capable of restricting intraphagosomal acidification and induces less apoptosis and autophagy. Importantly, the membrane of phagosomes enclosing the S forms showed signs of alteration such as breaks or partial degradation. Although not frequently encountered, these events suggest that the S form is capable of provoking phagosome-cytosol communications. In conclusion, M. abscessus S exhibits traits inside macrophages that are reminiscent of slow-growing mycobacterial species.
%I The Royal Society