Figure S4: Pol IV 21-22 nt siRNAs are enriched in AGO1 from <i>Arabidopsis</i> RNA polymerase IV generates 21–22 nucleotide small RNAs that can participate in RNA-directed DNA methylation and may regulate genes
Kaushik Panda
Andrea D. McCue
R. Keith Slotkin
10.6084/m9.figshare.11449989.v1
https://rs.figshare.com/articles/journal_contribution/Figure_S4_Pol_IV_21-22_nt_siRNAs_are_enriched_in_AGO1_from_i_Arabidopsis_i_RNA_polymerase_IV_generates_21_22_nucleotide_small_RNAs_that_can_participate_in_RNA-directed_DNA_methylation_and_may_regulate_genes/11449989
sRNA accumulation from different loci is shown in total, AGO1-IP or negative control mock-IP samples in wt Col inflorescence tissue. A) High enrichment of all miRNAs in AGO1-IP vs mock-IP. B) and C) Positive controls miR166 and miR171 [45] show high enrichment of specific mature miRNA in AGO1. D) miRNA 390a is known to be incoporated specifically into AGO7 and not AGO1 [45,46] and is shown here as a negative control demonstrating no enrichment of mature miRNA in AGO1-IP vs mock. E) Only a subset of 24 nt sRNA from Pol IV 24 nt clusters are incorporated into AGO1-IP (AGO1-IP/total < 0.2) but the ones that do are enriched compared to mock (AGO1-IP- /mock IP > 2). and F) 21-22 nt sRNAs from Pol IV 21-22 nt clusters show genome-wide enrichment into AGO1 (AGO1-IP/total > 2) like all miRNAs in A. AGO1-IP/mock enrichment (>3) is not as high as miRNAs but higher than the negative control miR390a. Together these data demonstrate that Pol IV-dependent 21-22 nt sRNAs are incorporated into AGO1.
2019-12-24 16:16:12
pollen
small interfering RNA
siRNA
RNA polymerase IV
pol IV
transposable element